Rictor is a central target of the molecular network of cardiac ProtectomiRs

Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main source(s): Research, Development and Innovation Office Hungary (NKFIA; NVKP-16-1-2016-0017 Heart Program OTKA-FK 134751); MTA-SE System Pharmacology Research Group, Department Pharmacotherapy, Semmelweis University, H-1089 Budapest, We have previously identified several cardiac microRNAs that are involved in cardioprotection termed them as ProtectomiRs. mRNA targets these ProtectomiRs may reveal new drug for cardioprotection. Here we aimed to identify key molecular confirm their association with a translational pig model acute myocardial infarction. Network theoretical approach was utilized 882 potential target genes 18 described protectomiRs. Rictor gene the most central it ranked first protectomiR-target network highest node degree 5. Therefore, expression its targeting were further validated heart samples obtained from infarction induced by pre- or postconditioning. Three out five Rictor-targeting homologue rat showed significant upregulation postconditioned but not preconditioned hearts. downregulated at protein level ischemic postconditioning preconditioning. This is demonstration decreased regulate postconditioning-, preconditioning-induced conclude novel